Tnfrsf1b Knockout cell line(MC38)

Tnfrsf1b Knockout cell line(MC38) Order

Catalog#

YKO-M012

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Tnfrsf1b Knockout cell line (MC38)

Morphology

Epithelial-like, Mixed: adherent and suspensio

Passage ratio

1：2-1：4

Species

Mouse

Gene Name

Tnfrsf1b

Gene ID

21938

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10%FBS

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:MC38			STR Info (Cell bank) Cell Line:MC38
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
4-2	237 【20.3】			237.89 【20.3】
5-5	343.75 【16】	347.53 【17】		344.16 【16】	348.22 【17】
6-4	299.55 【18】			300.21 【18】
6-7	345.94 【15】	349.92 【16】		346.68 【15】	350.59 【16】
9-2	232.81 【18】			233.55 【18】
12-1	228.95 【17】			230.17 【17】
15-3	200.44 【22.3】	204.44 【23.3】		201 【22.3】
18-3	148.15 【15】	152.13 【16】		148.28 【15】	152.32 【16】
X-1	407.91 【27】			409.18 【27】
D4S2408

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Tnfrsf1b gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MC38 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Tnfrsf1b gene knockout Tnfrsf1b Knockout cell line (MC38) will be expanded and cryopreserved.

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